Osong Public Health and Research Perspectives

Original Articles

A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis: Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi; Osong Public Health Res Perspect. 2017;8(1):65-70. Published online February 28, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09

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Objectives

Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods

All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014.

Results

Biochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis.

Conclusion

Quick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.

Citations

Citations to this article as recorded by

Development of a simplified and cost-effective sample preparation method for genotyping of human papillomavirus by next-generation sequencing
Rungrat Jitvaropas, Ukrit Thongpoom, Vorthon Sawaswong, Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Pornjarim Nilyanimit, Yong Poovorawan, Sunchai Payungporn
Archives of Virology.2023;[Epub] CrossRef
Bovine brucellosis – a comprehensive review
Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
Veterinary Quarterly.2021; 41(1): 61. CrossRef
Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
Nicolás Galarce, Sebastián de la Fuente, Beatriz Escobar, Phillip Dettleff, Pedro Abalos, Juan Carlos Hormazábal, Roberto Flores, Nicole Sallaberry-Pincheira, Víctor Martínez
Animals.2021; 11(7): 1980. CrossRef
Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis
Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam
Scientific Reports.2020;[Epub] CrossRef
Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani
Saudi Journal of Biological Sciences.2019; 26(2): 256. CrossRef
Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562. CrossRef
Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
Tina S. Lusk Pfefer, Ruth Timme, Julie A. Kase
Food Microbiology.2018; 70: 113. CrossRef

Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera: Reza Ranjbar, Ali Naghoni, Davoud Afshar, Farhad Nikkhahi, Mohsen Mohammadi; Osong Public Health Res Perspect. 2016;7(6):373-377. Published online December 31, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.10.002

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Abstract PDF

Objectives
Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera.
Methods
The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species.
Results
Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms.
Conclusion
Our study indicated that the invA, putative integrase, and ompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae.

Citations

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Development and Validation of an Efficient Multiplex PCR Assay for Simultaneous Detection of Six Common Foodborne Pathogens and Hygiene Indicators
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Rapid and multiplexed quantification of Salmonella, Escherichia coli O157:H7, and Shigella flexneri in ground beef using flow cytometry
Ziquan Wang, Qian Xu, Siyuan Liu, Yingying Liu, Ying Gao, Meng Wang, Ling Zhang, Haiyan Chang, Qiang Wei, Zhiwei Sui
Talanta.2022; 238: 123005. CrossRef
Development of multiplex real-time quantitative PCR for simultaneous detection of Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma urealyticum, and Mycoplasma genitalium in infertile women
Sara Sadeqi, Farhad Nikkhahi, Amir Javadi, Sonia Eskandarion, Seyed Mahmoud Amin Marashi
Indian Journal of Medical Microbiology.2022; 40(2): 231. CrossRef
Multiple fluorescent saltatory rolling circle amplification (SRCA) for simultaneous and sensitive detection of Salmonella spp. and Shigella spp. in food
Wei Guo, Qian Yang, Jie Liu, Xiuling Chen, Yunzhe Zhang, Wei Zhang
LWT.2022; 168: 113875. CrossRef
Campylobacter Species in the Middle East
Daryoush Babazadeh, Reza Ranjbar
Journal of Veterinary Physiology and Pathology.2022; 1(1): 1. CrossRef
Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera
Mohammad Lukman Yahaya, Nor Dyana Zakaria, Rahmah Noordin, Khairunisak Abdul Razak
Biotechnology and Applied Biochemistry.2021; 68(5): 1095. CrossRef
Ultrasensitive pathogen detection with a rolling circle amplification-empowered multiplex electrochemical DNA sensor
Cheryl S.Y. Yeap, Thanyarat Chaibun, Su Yin Lee, Bin Zhao, Yuan Jan, Chan La-o-vorakiat, Werasak Surareungchai, Shiping Song, Benchaporn Lertanantawong
Chemical Communications.2021; 57(91): 12155. CrossRef
Taqman hydrolysis probe application for Escherichia coli, Salmonella enterica, and Vibrio cholerae detection in surface and drinking water
Ahmed K. A. El-Sayed, Mohamed I. Abou-Dobara, Camelia A. Abdel-Malak, Amira A. E. El-Badaly
Journal of Water, Sanitation and Hygiene for Devel.2019; 9(3): 492. CrossRef
Pathways of healthcare and antibiotics use following reported gastrointestinal illness: a cross-sectional study in rural Anhui, China
Xing Rong Shen, Maomao Xie, Jing Chai, Rui Feng, Jing Cheng, Rong Liu, Paul Kadetz, DeBin Wang
BMJ Open.2019; 9(8): e030986. CrossRef
DNA Microarray for Rapid Detection and Identification of Food and Water Borne Bacteria: From Dry to Wet Lab
Reza Ranjbar, Payam Behzadi, Ali Najafi, Raheleh Roudi
The Open Microbiology Journal.2017; 11(1): 330. CrossRef

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